A study was carried out for production of a pigment : bluish purple, using a mutant Streptomyres californicus KS-89-7. The mutant was induced from Streptomyces californicus KS-89 with N-methyl-N -nitro-N-nitrosoquanidin( MNNG ). It was immobilized on an inert substance made of colloidal sillica and 3.5 % sodium alginate with 1 to 10 ratio. ´Me diameter of inert bead was 2mm, and number of immobilized mutant spore was approximately 1.0 x 10´/ml. It was packed in a column reactor and fermentation was conducted with a substrate made of soluble starch 1%, glycerol 1.0%, sodium glutamate 0.1
sodium nitrate 0.05%, L-prolin 0.025% and with some trace elements. The aeration for production of the pigment was 2.5m1/min with semi-continuous fermentation The pigment production reached at peak on 8 days of fermentation, and the mutant produced the pigment 1.8 times more than its parent strain with the maximum pigment production of 1.72g/ Q . The pigment production continued for 24 hours of fermentation, and at the end of the fermentation the mutant produced the pigment 1.52g/l.
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